Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-4-10
pubmed:abstractText
We studied the effects of human plasma lipoproteins on the synthesis of prostaglandin (PG) E2 in Swiss 3T3 mouse fibroblasts. Quiescent cells, maintained in medium deficient in both platelet-derived growth factor (PDGF) and lipoproteins, synthesized less than 8 ng of PGE2 per 10(6) cells per 22 hr, and this rate did not change in response to the addition of lipoproteins. In contrast, PDGF-stimulated cells, incubated in medium deficient in lipoproteins, synthesized 45-110 ng of PGE2 per 10(6) cells during the same period of time, and this rate increased 2- to 5-fold in the presence of added low density lipoproteins (LDL). This stimulatory effect of LDL seemed to depend on LDL receptor-mediated binding, uptake, and degradation of the lipoproteins because: both LDL and very low density lipoproteins were active, whereas high density lipoproteins were not; low concentrations of LDL were effective; the effect of native LDL was blocked by acetylation of the LDL; PDGF increased both the expression of LDL receptors and the cellular uptake of LDL; chloroquine blocked the effect of LDL but not that of exogenous arachidonic acid. These results provide evidence that the LDL pathway is critically linked to PG synthesis in PDGF-stimulated cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-13252080, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-170273, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-184464, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-3918038, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-3921570, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-3921636, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6254970, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6255471, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6261329, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6279599, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6297442, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6373049, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6768732, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6795201, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-6933457, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-7045092, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-7068680, http://linkedlifedata.com/resource/pubmed/commentcorrection/3081895-953168
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1344-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Low density lipoprotein receptor-dependent prostaglandin synthesis in Swiss 3T3 cells stimulated by platelet-derived growth factor.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't