Linked Life Data

3080434

Source:http://linkedlifedata.com/resource/pubmed/id/3080434

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-3-21
pubmed:abstractText
Monoclonal antibodies have been raised against a dimeric cell surface antigen (p75/150) which is specifically associated with the tumorigenic phenotype in human fibroblast X HeLa hybrids. During biosynthesis, a precursor molecule (p70/140), was associated with microsomal membranes in vivo but possessed no detectable cytoplasmic domains. At this stage, each p70 monomer contained 3 "high-mannose" type N-linked glycans which were subsequently processed into endoglycosidase H-insensitive complex oligosaccharides on the mature cell surface forms. Cleavage of this cell surface form with endoglycosidase F yielded non-N-glycosylated polypeptides of Mr = 60,000/120,000. All the monoclonal antibodies identified similar non-N-glycosylated polypeptides in cells grown in the presence of tunicamycin. p75/150 could be weakly labeled with [3H]palmitic or myristic acid. In vivo, p75/150 was found to be phosphorylated on serine residues. Immunoprecipitates of p75/150 from HeLa or tumorigenic hybrid cell lysates exhibited protein kinase activity in vitro, which phosphorylated p75/150 itself, also on serine residues. We were unable to detect this kinase activity in normal fibroblasts and in the nontumorigenic hybrid cells. Furthermore, we were unable to detect p75/150 or its precursors by either cell surface labeling, metabolic labeling, or Western blotting in nontumorigenic cell hybrids; p75/150 thus represents a tumor-specific marker in this system. Tryptic peptides of highly purified p75/150 have been generated, but their amino acid sequences did not reveal any significant homology with previously described proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
261
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2418-24
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:3080434-Amino Acid Sequence, pubmed-meshheading:3080434-Antibodies, Monoclonal, pubmed-meshheading:3080434-Antibodies, Neoplasm, pubmed-meshheading:3080434-Antigen-Antibody Complex, pubmed-meshheading:3080434-Antigens, Surface, pubmed-meshheading:3080434-Cell Transformation, Neoplastic, pubmed-meshheading:3080434-Fibroblasts, pubmed-meshheading:3080434-Glycoproteins, pubmed-meshheading:3080434-Glycoside Hydrolases, pubmed-meshheading:3080434-HeLa Cells, pubmed-meshheading:3080434-Humans, pubmed-meshheading:3080434-Hybrid Cells, pubmed-meshheading:3080434-Immunoelectrophoresis, pubmed-meshheading:3080434-Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, pubmed-meshheading:3080434-Peptides, pubmed-meshheading:3080434-Phosphoproteins, pubmed-meshheading:3080434-Phosphorylation, pubmed-meshheading:3080434-Protein Precursors, pubmed-meshheading:3080434-Protein Processing, Post-Translational, pubmed-meshheading:3080434-Tunicamycin
pubmed:year
1986
pubmed:articleTitle
Structural and functional features of a cell surface phosphoglycoprotein associated with tumorigenic phenotype in human fibroblast x HeLa cell hybrids.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't