Linked Life Data

3064767

Source:http://linkedlifedata.com/resource/pubmed/id/3064767

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-3-23
pubmed:abstractText
Tuna (Thunnus thynnus) growth hormone (GH) was purified by using a column of Sepharose 4B to which tuna GH-specific IgG was linked. The molecular weight and isoelectric point of tuna GH were 21,000 and 6.5, respectively. The growth of snapper (Pagrus major) was remarkably accelerated when the purified hormone was administered by four intraperitonial injections at intervals of 5 days: 1.5-fold in length and 1.9-fold in body weight/60 days. To produce tuna GH in Escherichia coli cells, expression plasmids pTES8 and pTES8S for tuna GH cDNA with or without the signal peptide region were constructed and GH production in E. coli cells was examined with the Maxicell system. The product specified by the plasmids in E. coli cells was immunologically identified to be tuna GH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0885-4513
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
385-93
pubmed:dateRevised
2007-3-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Growth-promoting activity of tuna growth hormone and expression of tuna growth hormone cDNA in Escherichia coli.
pubmed:affiliation
Institute for Research and Development, Taiyo Fisheries Co., Ltd., Tokyo, Japan.
pubmed:publicationType
Journal Article