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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-2-6
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pubmed:abstractText |
The avian acute leukemia virus E26 induces a mixed erythroid-myeloid leukemia in chicken and carries two distinct oncogenes, v-myb and v-ets. It was shown that there exist two ets loci in humans, c-ets-1 and c-ets-2, which were mapped to human chromosome 11 and 21, respectively. The human c-ets-1 proto-oncogene locus was shown to translocate from chromosome 11 to 9 or 4 in certain patients with acute monocytic leukemia. In order to understand the functional role of c-ets-1 proto-oncogene, we have made a cDNA library from MOLT-4 cells and isolated seven c-ets-1 positive cDNA clones. Analysis of two cDNA clones revealed a complete open reading frame of the c-ets-1 proto-oncogene. Except for 81 nucleotides at the 5'-end (downstream of initiation codon) and 39 nucleotides at the 3'-end (upstream of termination codon), the c-ets-1 cDNA is approximately 82% homologous to v-ets oncogene sequences and encodes a 441-residue protein (c-ets-1a) which contains contiguous 5' and 3' regions of the v-ets oncogene as in the case of chicken. Sequence analysis also revealed alternative splicing, giving rise to another related c-ets-1 polypeptide (c-ets-1b) lacking 87 amino acids. Our results suggest that differential RNA splicing allows the synthesis of at least two related c-ets-1 polypeptides from a single c-ets-1 proto-oncogene.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-ets,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/oncogene proteins v-ets
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pubmed:status |
MEDLINE
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pubmed:issn |
0890-6467
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-46
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3060801-Amino Acid Sequence,
pubmed-meshheading:3060801-Avian myeloblastosis virus,
pubmed-meshheading:3060801-Base Sequence,
pubmed-meshheading:3060801-DNA,
pubmed-meshheading:3060801-Gene Expression Regulation,
pubmed-meshheading:3060801-Humans,
pubmed-meshheading:3060801-Molecular Sequence Data,
pubmed-meshheading:3060801-Proto-Oncogene Proteins,
pubmed-meshheading:3060801-Proto-Oncogene Proteins c-ets,
pubmed-meshheading:3060801-Proto-Oncogenes,
pubmed-meshheading:3060801-RNA Splicing,
pubmed-meshheading:3060801-Retroviridae Proteins,
pubmed-meshheading:3060801-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:3060801-Sequence Homology, Nucleic Acid,
pubmed-meshheading:3060801-Transcription Factors,
pubmed-meshheading:3060801-Tumor Cells, Cultured
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pubmed:year |
1988
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pubmed:articleTitle |
Structure, expression and alternative splicing of the human c-ets-1 proto-oncogene.
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pubmed:affiliation |
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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