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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1988-12-14
|
| pubmed:abstractText |
Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor from bovine brain stimulate growth in a variety of tissues in several species. Despite the 55% amino acid sequence homology of the two forms of FGF, a specific immunoassay of aFGF has been developed using a polyclonal antibody raised in a rabbit. Two immunoassays were compared: a radioimmunoassay (RIA) using 125I aFGF and an enzyme immunoassay (EIA) using aFGF coupled to the tetrameric form of acetylcholinesterase (aFGF-AchE) as tracer. With EIA, the detection limit was 1.5 ng/ml, versus 2.2 ng/ml with RIA, while the dose at 50% was 5.9 ng/ml for EIA and 9.6 ng/ml for RIA. Using a modified EIA procedure where aFGF-AchE was added 2 h after the other reagents, the dose at 50% binding was 1.5 ng/ml. Examples of the performance of both immunoassays are presented for various brain extracts of different species including human. The aFGF content obtained by these methods correlates (CR = 0.987) with the values obtained by biological assay.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
173
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
328-39
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3056106-Acetylcholinesterase,
pubmed-meshheading:3056106-Animals,
pubmed-meshheading:3056106-Antibody Formation,
pubmed-meshheading:3056106-Antibody Specificity,
pubmed-meshheading:3056106-Cattle,
pubmed-meshheading:3056106-Cells, Cultured,
pubmed-meshheading:3056106-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3056106-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3056106-Female,
pubmed-meshheading:3056106-Fibroblast Growth Factors,
pubmed-meshheading:3056106-Immunization,
pubmed-meshheading:3056106-Immunoblotting,
pubmed-meshheading:3056106-Immunoenzyme Techniques,
pubmed-meshheading:3056106-Iodine Radioisotopes,
pubmed-meshheading:3056106-Mice,
pubmed-meshheading:3056106-Mitogens,
pubmed-meshheading:3056106-Rabbits,
pubmed-meshheading:3056106-Radioimmunoassay
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Development and testing of radio and enzyme immunoassays for acidic fibroblast growth factor (aFGF).
|
| pubmed:affiliation |
Laboratoire de Biotechnologie des Cellules Eucaryotes, Universite Paris XII, Creteil, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|