Linked Life Data

3049651

Source:http://linkedlifedata.com/resource/pubmed/id/3049651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1988-11-23
pubmed:abstractText
We initiated a comparative study of four methods of yeast inoculum preparation: a spectrophotometric method, the Wickerham card method, a hemacytometer method, and the Prompt inoculation system. The variability in inoculum size obtained when each method was applied to two strains each of Candida albicans, Candida tropicalis, Candida parapsilosis, Torulopsis glabrata, Cryptococcus neoformans, and Saccharomyces cerevisiae was analyzed in a single laboratory. Each method was performed in triplicate on the same day and on three separate days to provide estimates of within-day and between-day variations. Inoculum size was determined by viable colony counts. The greatest range of inoculum sizes was seen with the Wickerham card method. Viable counts ranged from 1.1 X 10(6) to 24.2 X 10(6) CFU/ml among the 12 yeast isolates. The greatest variation was observed with the Prompt system. Within-day coefficients of variation averaged 19% (range, 4 to 45%), and between-day coefficients of variation averaged 22% (range, 3 to 51%). Variation between laboratories was evaluated by comparing inoculum values obtained by each method in three different laboratories for two strains of C. albicans. The spectrophotometric method was the least variable and the Wickerham card and hemacytometer methods were the most variable methods between laboratories. The spectrophotometric method is recommended as the method of choice for preparation of a standardized inoculum suspension for susceptibility testing of yeasts.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-3318388, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-3326524, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-3517051, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-3674846, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-4598611, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-6322363, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-6343524, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-646347, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-6874902, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-6885994, http://linkedlifedata.com/resource/pubmed/commentcorrection/3049651-7416742
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1437-41
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Multicenter evaluation of four methods of yeast inoculum preparation.
pubmed:affiliation
Veterans Administration Medical Center, Iowa City, Iowa 52242.
pubmed:publicationType
Journal Article, Clinical Trial, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Multicenter Study