Linked Life Data

3041409

Source:http://linkedlifedata.com/resource/pubmed/id/3041409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1988-9-7
pubmed:abstractText
Transcription of the yeast GAL1 and GAL10 genes is induced by growth on galactose. Using the technique of photofootprinting in vivo, we previously documented equivalent transcription-dependent footprints within the putative "TATA" elements of both genes. To explore the functional significance of these observations, we created a 3-base-pair substitution mutation within the GAL1 promoter TATA element, which disrupted the ATATAA consensus sequence but left intact the photomodification targets. The mutation reduced galactose-induced RNA levels by a factor of 100. The mutant promoter no longer displayed the characteristic TATA sequence footprint, supporting the hypothesis that transcription activation involves the binding of a TATA box factor. We also observed a collection of transcription-correlated alterations in the modification pattern at sites between the UASG and the GAL1 TATA element, within sequences that are not required for inducible transcription. These patterns, characteristic of the induced wild-type GAL1 gene, were still galactose inducible with the TATA mutant GAl1 promoter, despite the low level of transcription from this promoter. We conclude that the GAL4-dependent protein/DNA structure responsible for the altered pattern within nonessential sequences is therefore not strictly coupled to an active TATA element or to high levels of expression. Nonetheless, the patterns probably reflect a stable protein-dependent structure that accompanies assembly of the transcription initiation complex.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-2419896, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-265521, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-2881299, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-2882858, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-2943018, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-2985609, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3001709, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3009831, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3018583, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3115591, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3297349, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3297350, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3310730, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3313011, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3316976, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3527621, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3540601, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3543694, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3881758, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3886158, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-3909147, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-4075392, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-448736, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6092912, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6248234, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6276569, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6313211, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6323249, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6345791, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6390181, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6392852, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6537904, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6728031, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6760197, http://linkedlifedata.com/resource/pubmed/commentcorrection/3041409-6791577
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5399-403
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
In vivo "photofootprint" changes at sequences between the yeast GAL1 upstream activating sequence and "TATA" element require activated GAL4 protein but not a functional TATA element.
pubmed:affiliation
Department of Biological Chemistry, Washington University School of Medicine, Saint Louis, MO 63110.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't