|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1987-10-19
|
|
pubmed:abstractText |
We have purified a 52 kd protein, AP-2, that binds to enhancer regions of SV40 and human metallothionein IIA (hMT IIA) and stimulates RNA synthesis from these promoters in vitro. Surprisingly, AP-2 also binds to two SV40 early promoter regions recognized by Sp1 and T antigen. Juxtaposed binding sites for AP-2 and Sp1 in the 21 bp repeats may facilitate productive interactions between the two factors. In contrast, sequence-specific binding of AP-2 to SV40 and hMT IIA DNA is inhibited by the viral repressor protein T antigen. Furthermore, T antigen inhibits AP-2-dependent transcriptional activation of the hMT IIA promoter in vitro. The inhibition is neither a direct nor an indirect result of T antigen binding to DNA, because the hMT IIA promoter lacks T antigen binding sites. Instead, sedimentation studies suggest that protein-protein interactions between AP-2 and T antigen block AP-2 binding to DNA. These findings suggest novel mechanisms for mediating positive and negative regulation of transcription.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral, Tumor,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
50
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
847-61
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:3040262-Antigens, Polyomavirus Transforming,
pubmed-meshheading:3040262-Antigens, Viral, Tumor,
pubmed-meshheading:3040262-Base Sequence,
pubmed-meshheading:3040262-Binding Sites,
pubmed-meshheading:3040262-DNA, Viral,
pubmed-meshheading:3040262-DNA-Binding Proteins,
pubmed-meshheading:3040262-Enhancer Elements, Genetic,
pubmed-meshheading:3040262-Gene Expression Regulation,
pubmed-meshheading:3040262-Genes, Regulator,
pubmed-meshheading:3040262-Genes, Viral,
pubmed-meshheading:3040262-Humans,
pubmed-meshheading:3040262-Metallothionein,
pubmed-meshheading:3040262-Models, Genetic,
pubmed-meshheading:3040262-Oncogene Proteins, Viral,
pubmed-meshheading:3040262-Promoter Regions, Genetic,
pubmed-meshheading:3040262-Protein Binding,
pubmed-meshheading:3040262-Simian virus 40,
pubmed-meshheading:3040262-Transcription, Genetic,
pubmed-meshheading:3040262-Transcription Factor AP-2,
pubmed-meshheading:3040262-Transcription Factors
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
