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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1987-8-20
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pubmed:abstractText |
Rat aortic smooth muscle homogenates and membrane preparations contain a phospholipase C which hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2). We discovered that guanyl-5'yl-imidodiphosphate (Gpp(NH)p) activated the hydrolysis of exogenous PIP2 but not of phosphatidylinositol (PI) in rat aortic membranes. Further, maximal Gpp(NH)p-dependent hydrolysis was dependent on physiological levels of calcium. Also, magnesium inhibited PIP2 hydrolysis and catalyzed the dephosphorylation of PIP2 to phosphatidylinositol-4-phosphate (PIP). The results imply that PIP2 is the primary substrate of the nucleotide-regulated phospholipase C in rat aorta and that calcium and magnesium are physiological regulators of this activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
629-34
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3037230-Animals,
pubmed-meshheading:3037230-Aorta, Thoracic,
pubmed-meshheading:3037230-Calcium,
pubmed-meshheading:3037230-Cell Membrane,
pubmed-meshheading:3037230-Egtazic Acid,
pubmed-meshheading:3037230-Guanosine Triphosphate,
pubmed-meshheading:3037230-Guanylyl Imidodiphosphate,
pubmed-meshheading:3037230-Kinetics,
pubmed-meshheading:3037230-Magnesium,
pubmed-meshheading:3037230-Muscle, Smooth, Vascular,
pubmed-meshheading:3037230-Phosphatidylinositol 4,5-Diphosphate,
pubmed-meshheading:3037230-Phosphatidylinositols,
pubmed-meshheading:3037230-Rats,
pubmed-meshheading:3037230-Type C Phospholipases
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pubmed:year |
1987
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pubmed:articleTitle |
Modulation of phosphatidylinositol-4,5-bisphosphate hydrolysis in rat aorta by guanine nucleotides, calcium and magnesium.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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