|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0026882,
umls-concept:C0039649,
umls-concept:C0127400,
umls-concept:C0185117,
umls-concept:C0317568,
umls-concept:C1274040,
umls-concept:C1336789,
umls-concept:C2911684
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1987-4-6
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The Tn10-like constitutively expressed tetracycline resistance determinant from a Haemophilus parainfluenzae strain was cloned in Escherichia coli. Toxicity resulting from expression on multicopy plasmids necessitated its being cloned on a low-copy plasmid vector or in cells containing the Tn10-encoded repressor. Constitutive expression of tetracycline resistance was found to result from the synthesis of a truncated inactive repressor molecule. Instead of the 23-kilodalton repressor found in other Tn10-containing strains, this determinant encoded a 14.5-kilodalton molecule. The DNA sequence of the 700-base-pair region spanning the repressor gene and promoter-operator regions of the Haemophilus determinant was identical to that of the same region of Tn10, except for the absence of a single T X A base pair in the repressor gene. This deletion leads to premature termination of the protein. Antisera to the repressor suggested that the repressor was also absent in a second independently isolated H. parainfluenzae strain bearing a Tn10-like constitutive tetracycline resistance determinant.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-1096670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-372049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-372978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6090437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6093049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6229640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6271633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6282805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6298728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6307983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6311683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6317652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6325175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6330236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6330687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6660856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6788377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6801018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3029042-6927764
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0021-9193
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
169
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
990-4
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3029042-Cloning, Molecular,
pubmed-meshheading:3029042-DNA Transposable Elements,
pubmed-meshheading:3029042-Drug Resistance, Microbial,
pubmed-meshheading:3029042-Genes,
pubmed-meshheading:3029042-Genes, Bacterial,
pubmed-meshheading:3029042-Haemophilus,
pubmed-meshheading:3029042-Mutation,
pubmed-meshheading:3029042-Plasmids,
pubmed-meshheading:3029042-Repressor Proteins,
pubmed-meshheading:3029042-Tetracycline,
pubmed-meshheading:3029042-Transcription Factors
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
