Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-12-29
pubmed:abstractText
We have analyzed the effect of insertion mutants between the simian virus 40 (SV40) 21-base pair (bp) repeats and the early-early (EE) TATA sequence. Insertion of 4, 42, or 90 bp of DNA at the SV40 NcoI site (map position 37) has been analyzed for its effect on expression of the SV40 early gene and positioning of the RNA 5' ends. Insertion of 4 bp reduced SV40 early promoter-dependent chloramphenicol acetyltransferase (CAT) expression by six- to eightfold. Increasing the size of the insertion to 42 or 90 bp resulted in a further drop in early gene expression to basal levels. At the RNA level, the 4-bp insertion reduced EE RNA synthesis approximately 10-fold. No concomitant increase in late-early (LE) RNA synthesis was observed. Insertion of 42 or 90 bp of DNA resulted in a decrease of EE RNA synthesis and a stimulation of LE RNA synthesis. Deletion of the SV40 72-bp repeats from the insertion mutants demonstrated that some, but not all, of the LE RNA depends upon the presence of these sequences. These studies suggest that the ability of RNA polymerase II to utilize the EE (TATA-directed) transcriptional control sequence requires an interaction with the upstream 21-bp repeats or the 72-bp repeats or both. That LE RNA levels in pJI1-in42 CAT and pJI1-in90 CAT were equivalent to the level of EE RNA in pJI1-CAT, yet the level of CAT gene expression was decreased greater than 10-fold, suggests that LE mRNA is under translational control and probably prefers a 5' initiation codon proximal to that of the CAT gene.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-11892819, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-2992804, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-2993862, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-2997782, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-3004974, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-4041012, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-4075392, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-4075400, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6092914, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6092946, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6092947, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6098465, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6098828, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6194902, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6233005, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6259538, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6262784, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6264425, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6270376, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6273820, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6277502, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6296253, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6304653, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6319019, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6319021, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6320125, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6321158, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6409419, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6537904, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6828151, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6828386, http://linkedlifedata.com/resource/pubmed/commentcorrection/3023682-6960240
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
935-42
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Spacing between simian virus 40 early transcriptional control sequences is important for regulation of early RNA synthesis and gene expression.
pubmed:publicationType
Journal Article