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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
17
|
| pubmed:dateCreated |
1986-11-24
|
| pubmed:abstractText |
Polymorphonuclear leukocytes (PMNLs) store collagenase in an inactive form in secretory granules. The enzyme can be activated in vitro by limited proteolysis or by sulfhydryl-modifying agents such as N-ethylmaleimide (NEM). We have enriched NEM-activated collagenase 820-fold using granule isolation, gel filtration, and wheat germ agglutinin (WGA)-agarose chromatography. The use of WGA-agarose resulted in a 55-fold enrichment of collagenase in a single step with very little loss of activity. The chromatographic behavior of collagenase on other lectin matrices was explored and gave information about the type of complex asparagine-linked oligosaccharide found on collagenase isolated from PMNLs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-2960
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
26
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4757-62
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3021203-Chromatography, Affinity,
pubmed-meshheading:3021203-Enzyme Activation,
pubmed-meshheading:3021203-Ethylmaleimide,
pubmed-meshheading:3021203-Humans,
pubmed-meshheading:3021203-Kinetics,
pubmed-meshheading:3021203-Microbial Collagenase,
pubmed-meshheading:3021203-Molecular Weight,
pubmed-meshheading:3021203-Neutrophils,
pubmed-meshheading:3021203-Wheat Germ Agglutinins
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Use of lectin affinity chromatography for the purification of collagenase from human polymorphonuclear leukocytes.
|
| pubmed:publicationType |
Journal Article
|