rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
8
|
pubmed:dateCreated |
1986-11-17
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pubmed:abstractText |
Large tumor antigen (T antigen) was extracted from SV40-infected African Green Monkey cells and purified to homogeneity by immunoaffinity chromatography. The purified T antigen preparations unwind DNA duplices of greater than 120 bp in a reaction which is dependent on magnesium ions and ATP hydrolysis. Based on these and other properties of the reaction we classify this newly discovered enzymatic activity as a eukaryotic DNA helicase. The helicase and the known ATPase function of T antigen cosediment with the mono- or dimeric 4-6 S form of T antigen, but not with higher T antigen aggregates. The helicase activity seems to be an intrinsic function of SV40 T antigen. First, several different T antigen-specific monoclonal antibodies interfere with the DNA unwinding activity; monoclonals which are known to reduce the T antigen-specific ATPase most strongly inhibited the helicase reaction. Second, mutant T antigens with impaired ATPase function also showed a reduced DNA unwinding activity.
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pubmed:commentsCorrections |
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
|
pubmed:issn |
0261-4189
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
5
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
1939-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3019672-Animals,
pubmed-meshheading:3019672-Antigens, Polyomavirus Transforming,
pubmed-meshheading:3019672-Antigens, Viral, Tumor,
pubmed-meshheading:3019672-Cell Line,
pubmed-meshheading:3019672-Cercopithecus aethiops,
pubmed-meshheading:3019672-DNA Topoisomerases, Type I,
pubmed-meshheading:3019672-Kidney,
pubmed-meshheading:3019672-Kinetics,
pubmed-meshheading:3019672-Oncogene Proteins, Viral,
pubmed-meshheading:3019672-Simian virus 40
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pubmed:year |
1986
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pubmed:articleTitle |
DNA helicase activity of SV40 large tumor antigen.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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