| pubmed-article:3017794 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3017794 | lifeskim:mentions | umls-concept:C0085455 | lld:lifeskim |
| pubmed-article:3017794 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:3017794 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:3017794 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:3017794 | lifeskim:mentions | umls-concept:C2348867 | lld:lifeskim |
| pubmed-article:3017794 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3017794 | pubmed:dateCreated | 1986-10-22 | lld:pubmed |
| pubmed-article:3017794 | pubmed:abstractText | Tn5 lac is a transposon that fuses the transcription of lacZ to exogenous promoters. We generated 2374 Tn5 lac insertion-containing strains of Myxococcus xanthus, a soil bacterium that undergoes multicellular development which culminates in the formation of spores. Thirty-six strains were identified that specifically increase beta-galactosidase expression at some particular time during development and these expression times range from minutes after starvation initiates development to 24 hr, when sporulation begins. Different maximum levels of beta-galactosidase expression were also observed and the maximum for many strains that begin beta-galactosidase expression late in development was observed only if spores were disrupted. Seven of the 36 strains display mild to severe defects in aggregation and/or sporulation, as did an additional five strains whose beta-galactosidase expression was not developmentally regulated. Restriction maps of the DNA adjacent to the Tn5 lac insertions that are developmentally regulated and/or cause developmental defects show that most of the 41 insertions are in different regions of the Myxococcus genome. The developmentally regulated Tn5 lac insertions described here provide a set of at least 29 new developmental markers for Myxococcus. | lld:pubmed |
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| pubmed-article:3017794 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3017794 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3017794 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3017794 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3017794 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3017794 | pubmed:issn | 0012-1606 | lld:pubmed |
| pubmed-article:3017794 | pubmed:author | pubmed-author:KaiserDD | lld:pubmed |
| pubmed-article:3017794 | pubmed:author | pubmed-author:KuspaAA | lld:pubmed |
| pubmed-article:3017794 | pubmed:author | pubmed-author:KroonGG | lld:pubmed |
| pubmed-article:3017794 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3017794 | pubmed:volume | 117 | lld:pubmed |
| pubmed-article:3017794 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3017794 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3017794 | pubmed:pagination | 252-66 | lld:pubmed |
| pubmed-article:3017794 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:3017794 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3017794 | pubmed:articleTitle | A global analysis of developmentally regulated genes in Myxococcus xanthus. | lld:pubmed |
| pubmed-article:3017794 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3017794 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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