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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-10-22
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pubmed:abstractText |
Tn5 lac is a transposon that fuses the transcription of lacZ to exogenous promoters. We generated 2374 Tn5 lac insertion-containing strains of Myxococcus xanthus, a soil bacterium that undergoes multicellular development which culminates in the formation of spores. Thirty-six strains were identified that specifically increase beta-galactosidase expression at some particular time during development and these expression times range from minutes after starvation initiates development to 24 hr, when sporulation begins. Different maximum levels of beta-galactosidase expression were also observed and the maximum for many strains that begin beta-galactosidase expression late in development was observed only if spores were disrupted. Seven of the 36 strains display mild to severe defects in aggregation and/or sporulation, as did an additional five strains whose beta-galactosidase expression was not developmentally regulated. Restriction maps of the DNA adjacent to the Tn5 lac insertions that are developmentally regulated and/or cause developmental defects show that most of the 41 insertions are in different regions of the Myxococcus genome. The developmentally regulated Tn5 lac insertions described here provide a set of at least 29 new developmental markers for Myxococcus.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0012-1606
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
252-66
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3017794-Bacterial Proteins,
pubmed-meshheading:3017794-Cell Differentiation,
pubmed-meshheading:3017794-Chromosome Mapping,
pubmed-meshheading:3017794-Cloning, Molecular,
pubmed-meshheading:3017794-DNA, Bacterial,
pubmed-meshheading:3017794-DNA Restriction Enzymes,
pubmed-meshheading:3017794-DNA Transposable Elements,
pubmed-meshheading:3017794-Gene Expression Regulation,
pubmed-meshheading:3017794-Genes, Bacterial,
pubmed-meshheading:3017794-Myxococcales,
pubmed-meshheading:3017794-Promoter Regions, Genetic,
pubmed-meshheading:3017794-Spores, Bacterial,
pubmed-meshheading:3017794-Time Factors
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pubmed:year |
1986
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pubmed:articleTitle |
A global analysis of developmentally regulated genes in Myxococcus xanthus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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