Linked Life Data

3011932

Source:http://linkedlifedata.com/resource/pubmed/id/3011932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1986-6-30
pubmed:abstractText
The oxidative response of rat polymorphonuclear leukocytes stimulated by phorbol myristate acetate and N-formyl-methionyl-leucyl-phenylalanine was studied. Ferricytochrome reduction and peroxidase-catalyzed decrease of scopoletin fluorescence were used to monitor O-2 and H2O2 release in the extracellular medium. Oxygen consumption was also measured in some experiments. Decrease of chlortetracyclin fluorescence after stimulation of dye-loaded cells was used to study an early step of cell stimulation. Finally, a possible relationship between cell responses and the medium redox potential was explored. Three major conclusions were obtained: Ferricytochrome reduction is dependent on the total cytochrome concentration, and a simple mathematical model allows a tentative estimate of total superoxide anion production by stimulated cells. Increasing cell concentration results in a decrease of individual cell response, and this may be accounted for by a direct inhibition of cell-released hydrogen peroxide on the reactivity of leukocytes. Further, H2O2 may be shown to inhibit an early step of cell response. The solution redox potential does not influence cell reactivity, since it may be dramatically decreased without inhibiting cell response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0741-5400
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
599-616
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Self-limitation of the oxidative burst of rat polymorphonuclear leukocytes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't