Linked Life Data

3010076

Source:http://linkedlifedata.com/resource/pubmed/id/3010076

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-6-23
pubmed:abstractText
Uptake of paraquat (PQ; 10 microM) into lung cell fractions enriched in alveolar type II cells or Clara cells was linear with time, and after 60 min the intracellular concentration was approximately 10-fold higher than that in the medium. In contrast, alveolar macrophages were not able to accumulate PQ from the extracellular medium. PQ uptake in preparations of type II and Clara cells, but not alveolar macrophages, was inhibited by an equimolar concentration of putrescine or spermidine and by a combination of the metabolic inhibitors, potassium cyanide and iodoacetate (1 mM each). The reduction of PQ (1 mM) under anaerobic conditions was investigated in lung cells by ESR spectroscopy. The amplitude of the ESR signal of the PQ radical increased with time with intact or sonicated type II and Clara cell preparations, but with macrophages it increased only when the cells were sonicated. The signal in sonicated cells but not whole cells was decreased by addition of antibodies to NADPH-cytochrome P-450 reductase, suggesting that the PQ radical is generated intracellularly under these conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
484-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Paraquat uptake into freshly isolated rabbit lung epithelial cells and its reduction to the paraquat radical under anaerobic conditions.
pubmed:publicationType
Journal Article, In Vitro