Linked Life Data

3009490

Source:http://linkedlifedata.com/resource/pubmed/id/3009490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-6-12
pubmed:abstractText
Insertion of a crude preparation of cyclic AMP (cAMP)-dependent protein kinase inhibitor (PKI) into a cloned mouse anterior pituitary cell line (AtT-20/D16-16) blocked cAMP-mediated hormone release. This was accomplished by developing a technique to incorporate PKI into multicellular cultures. The technique involved the encapsulation of the PKI into liposomes coupled to Protein A (a bacterial protein that binds to the Fc portion of antibodies). Application of such liposomes to AtT-20 cells targeted by pre-treatment with an antiserum against neural cell adhesion molecule (a cell surface glycoprotein expressed by these cells) resulted in the attachment of the liposomes onto the cell surface followed by the delivery of the liposome content into the cells. The AtT-20 cells respond to cAMP-promoting agents such as forskolin by secreting the hormone adrenocorticotropin (ACTH). Liposomes containing PKI and coupled to protein A specifically blocked cAMP-mediated ACTH release from cells treated with anti-N-CAM antibodies. In contrast, the ACTH release response to K+ or phorbol esters does not appear to involve cAMP and was not reduced by such manipulations. The specificity of PKI to block hormone release initiated by one but not by other secretagogues directly links cAMP-dependent protein kinase with the ACTH release process but suggests that there are other mechanisms also involved in stimulus-secretion coupling in corticotrophs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-17552, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-180015, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-210798, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-279908, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6088493, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6092042, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6109620, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6111794, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6134615, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6142943, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6143403, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6144727, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6232463, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6261262, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6276162, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6288045, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6292240, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6298209, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6300893, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6305967, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6333336, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6355895, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6615464, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6745267, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6849906, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6877355, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-6954530, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-7031274, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-7051006, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-7188249, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-7278980, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-7316186, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-7442804, http://linkedlifedata.com/resource/pubmed/commentcorrection/3009490-95096
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone, http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Forskolin, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/Liposomes, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Staphylococcal Protein A, http://linkedlifedata.com/resource/pubmed/chemical/protein kinase modulator
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1630-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...