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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-4-16
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pubmed:abstractText |
The fractionation abilities of several ion-exchangers of the high-performance liquid chromatography type for two sets of DNA restriction fragments, ranging from 7 base pairs (bp) to about 650 bp and differing in their mean base composition, have been studied. The ion-exchangers tested comprise the RPC-5, the 5-PW DEAE and the Mono Q as polymer-based resins, and the Nucleogens 500 and 4000, both prepared from silica beads. The results indicate that all the ion-exchangers except the 5-PW DEAE perfectly separate fragment sizes up to about 90 bp, the 5-PW DEAE separating to 45 bp only. Above 200 bp only the Mono Q resin works in a satisfactory way provided that about 100 micrograms DNA mixture, containing less than 25 fragments within the given size range, is loaded per milliliter of packed resin. Appreciable base-pair specificities were detected for most of the resins which cause substantial retardations of the d(A + T)-rich fragments with respect to the eluting salt concentration. If the latter dominate in the DNA sample, acceptable results were only obtained with the Mono Q resin when the column was operated at elevated temperature.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
203-12
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:3004989-Base Composition,
pubmed-meshheading:3004989-Chromatography, High Pressure Liquid,
pubmed-meshheading:3004989-DNA,
pubmed-meshheading:3004989-DNA Restriction Enzymes,
pubmed-meshheading:3004989-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3004989-Ion Exchange Resins,
pubmed-meshheading:3004989-Sodium Chloride,
pubmed-meshheading:3004989-Temperature
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pubmed:year |
1986
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pubmed:articleTitle |
Fractionation of DNA restriction fragments with ion-exchangers for high-performance liquid chromatography.
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pubmed:publicationType |
Journal Article
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