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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1986-3-14
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pubmed:abstractText |
A PyrF- mutant of Escherichia coli (SK1108, pyrF::Tn5 Kanr) was complemented with the Desulfovibrio vulgaris (Hildenborough) structural gene for orotidine-5'-phosphate decarboxylase (EC 4.1.1.23). Either orientation of a 1.6-kilobase-pair D. vulgaris DNA fragment (pLP3B or pLP3A) complemented the PyrF- strain suggesting that the D. vulgaris pyrF promoter was functional. The apparent product of the D. vulgaris pyrF gene was a single 26-kilodalton polypeptide. These results demonstrate the utility of E. coli cloning systems in studying metabolic and energetic pathways in sulfate-reducing bacteria.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-14336080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-284385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-368040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-3888620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-4612007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-4939624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-5009760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-6127735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-6319231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3003034-6355062
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
644-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3003034-Carboxy-Lyases,
pubmed-meshheading:3003034-Chromosome Mapping,
pubmed-meshheading:3003034-DNA, Bacterial,
pubmed-meshheading:3003034-DNA Restriction Enzymes,
pubmed-meshheading:3003034-Desulfovibrio,
pubmed-meshheading:3003034-Escherichia coli,
pubmed-meshheading:3003034-Genes, Bacterial,
pubmed-meshheading:3003034-Genetic Complementation Test,
pubmed-meshheading:3003034-Orotidine-5'-Phosphate Decarboxylase,
pubmed-meshheading:3003034-Transformation, Genetic
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pubmed:year |
1986
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pubmed:articleTitle |
Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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