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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1986-1-9
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pubmed:abstractText |
Approximately 80% of the genome of feline panleukopenia virus was cloned into the plasmid pBR322. The entire 3943 nucleotide sequence of the cloned portion of FPV was determined. This DNA includes the gene which codes for the structural proteins of the virus. Portions of this gene were expressed in E. coli as fusion proteins with bacterial proteins. Some of the fusion proteins were capable of raising neutralizing antibodies in guinea pigs. Through the use of deletion mapping, monoclonal antibodies, and synthetic peptides, attempts were made to localize the portion of the protein responsible for raising these antibodies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0065-2598
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3000149-Animals,
pubmed-meshheading:3000149-Antibodies, Monoclonal,
pubmed-meshheading:3000149-Antibodies, Viral,
pubmed-meshheading:3000149-Antigens, Viral,
pubmed-meshheading:3000149-Cats,
pubmed-meshheading:3000149-Cloning, Molecular,
pubmed-meshheading:3000149-DNA Restriction Enzymes,
pubmed-meshheading:3000149-Escherichia coli,
pubmed-meshheading:3000149-Feline panleukopenia virus,
pubmed-meshheading:3000149-Genes, Viral,
pubmed-meshheading:3000149-Guinea Pigs,
pubmed-meshheading:3000149-Neutralization Tests,
pubmed-meshheading:3000149-Oligopeptides,
pubmed-meshheading:3000149-Parvoviridae,
pubmed-meshheading:3000149-Viral Proteins,
pubmed-meshheading:3000149-Viral Vaccines
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pubmed:year |
1985
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pubmed:articleTitle |
The structural proteins of the autonomous parvovirus feline panleukopenia virus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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