Linked Life Data

2995999

Source:http://linkedlifedata.com/resource/pubmed/id/2995999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1985-11-18
pubmed:abstractText
The sequence analysis of both products of individual phi 80 site-specific recombination events in vivo shows that recombination with a secondary attachment (att) site generates several different novel joints at the mismatched position: one recombination event resulted in a single base-pair deletion and two other recombination events resulted in two different single base-pair substitutions. The characterized products of recombination can be straightforwardly interpreted as the outcome of strand exchange involving staggered nicks bracketing the heterology within an overlap region of five to nine base pairs. In comparison, more complex segregation patterns have been observed in previous studies of lambda recombination between nonidentical att sites; the nature of the overlap region heterology may have a significant effect on the segregation patterns. To recover both products of a single recombination event, we used a plasmid that carries the phi 80 int and xis genes and both att sites. Because the two att sites are situated in opposite orientation, intramolecular recombination between them inverts rather than deletes the intervening segment of DNA. Although subsequent reinversion restores the original gross genetic arrangement, single base-pair insertions, deletions, and substitutions are introduced at the sites of recombination. One of the mutations improves the recombination efficiency of the secondary att site and thereby converts a formerly "stable" sequence to an efficient target for rearrangement, and other mutations are predicted to alter the specificity of recombination. These pathways may also provide useful models for the efficient generation of localized sequence diversity on a development (as well as evolutionary) time scale.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-1069303, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-2984205, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-3159904, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-4778806, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6096022, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6099255, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6214637, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6226804, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6235918, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6241556, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6246368, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6305924, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6317202, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6457725, http://linkedlifedata.com/resource/pubmed/commentcorrection/2995999-6461289
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6990-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Generation of single base-pair deletions, insertions, and substitutions by a site-specific recombination system.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't