Linked Life Data

2995250

Source:http://linkedlifedata.com/resource/pubmed/id/2995250

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-11-12
pubmed:abstractText
In the mouse three lymphocyte gene families have been positioned on the proximal region of chromosome 6. Originally the immunoglobulin kappa light chain (Igk) and the thymocyte surface antigens Lyt-2 and Lyt-3 were assigned to chromosome 6, and recently the beta chain of the T-cell receptor for antigen was positioned proximal to Igk. Molecular clones which recognize the constant (C) region of the beta chain of the T-cell receptor for antigen (CT beta) and the constant region of the immunoglobulin kappa (C kappa) chain were used to determine recombination frequencies with respect to the morphological marker hypodactyly (Hd). SJL/JL W pi mice were mated with C.B6.C3-Hd/+ mice, and the progeny expressing the Hd phenotype were mated with SJL/JL W pi mice. Backcross progeny which expressed the Hd phenotype were nephrectomized, and kidney DNA was examined by Southern hybridization for the polymorphic restriction endonuclease fragment (REF) patterns of the parental mice. Of the 88 progeny tested in this three-point cross, 3 CT beta and 4 C kappa homozygote REF patterns were detected. These homozygotes were mutually exclusive. This implies the following gene order: centromere-CT beta-Hd-Igk and CT beta 1 would be 7.95 +/- 2.88 centimorgans from C kappa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0093-7711
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-83
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Localization of CT beta and C kappa on mouse chromosome 6.
pubmed:publicationType
Journal Article