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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1985-10-21
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pubmed:databankReference |
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pubmed:abstractText |
DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-228250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-274719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-351191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-4559594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-4939175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-5033417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-542126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6083565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6091124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6173769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6194404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6198242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6209582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6272997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6392892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6782162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-698046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6994549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-6997135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-7350544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-803646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-811672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-822353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-911786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2994016-92371
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5869-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2994016-Amino Acid Sequence,
pubmed-meshheading:2994016-Animals,
pubmed-meshheading:2994016-Base Sequence,
pubmed-meshheading:2994016-Cloning, Molecular,
pubmed-meshheading:2994016-DNA,
pubmed-meshheading:2994016-DNA Restriction Enzymes,
pubmed-meshheading:2994016-Escherichia coli,
pubmed-meshheading:2994016-Humans,
pubmed-meshheading:2994016-Interleukin-1,
pubmed-meshheading:2994016-Macrophages,
pubmed-meshheading:2994016-Nucleic Acid Hybridization,
pubmed-meshheading:2994016-Plasmids,
pubmed-meshheading:2994016-Protein Precursors,
pubmed-meshheading:2994016-RNA, Messenger,
pubmed-meshheading:2994016-Rabbits,
pubmed-meshheading:2994016-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.
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pubmed:publicationType |
Journal Article,
Comparative Study
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