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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-9-4
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pubmed:abstractText |
A protonated form of a cloned simple repeating DNA sequence d(TC)n X d(GA) is detectable in equilibrium with the usual Watson-Crick base-paired form at pHs up to 7. This form is anomalously sensitive to a variety of single-strand-specific endonucleases. The observed pH dependent protection of N-7 of dG residues within the insert suggests that these residues are either Hoogsteen or reverse Hoogsteen base-paired to protonated dC residues of the polypyrimidine strand. A structure in which dA:dT Watson-Crick base pairs alternate with Hoogsteen syndG:dCH+ pairs appears to be the most stereochemically acceptable structure consistent with the chemical properties of this protonated DNA. Protonated d(TC)n X d(GA)n interacts with an anti-Z DNA antibody raised against brominated d(GC)n X d(GC)n.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
271-80
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2990727-Cloning, Molecular,
pubmed-meshheading:2990727-DNA,
pubmed-meshheading:2990727-DNA, Superhelical,
pubmed-meshheading:2990727-Endodeoxyribonucleases,
pubmed-meshheading:2990727-Endonucleases,
pubmed-meshheading:2990727-Hydrogen-Ion Concentration,
pubmed-meshheading:2990727-Magnesium,
pubmed-meshheading:2990727-Nucleic Acid Conformation,
pubmed-meshheading:2990727-Plasmids,
pubmed-meshheading:2990727-Protons,
pubmed-meshheading:2990727-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2990727-Single-Strand Specific DNA and RNA Endonucleases
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pubmed:year |
1985
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pubmed:articleTitle |
A structural basis for S1 nuclease sensitivity of double-stranded DNA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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