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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1985-9-4
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pubmed:abstractText |
We have mapped a transcriptional regulatory sequence within the 1.0 kb noncoding region of the bovine papilloma virus (BPV-1) genome, using an enhancer dependent expression vector for chloramphenicol acetyltransferase. This transcriptional regulatory element works independently of position and orientation, and its function is significantly augmented in BPV-1 transformed C127 cells and in monkey CV-1 cells acutely transfected with plasmids expressing BPV-1 early gene products. Using defined deletion mutants of the BPV-1 DNA and full-length viral cDNAs expressed from an SV40 early promoter, we demonstrate that the expression of this trans-activating factor maps to the 3' open reading frames of the viral transforming region. A premature termination codon engineered into the E2 ORF eliminates expression of this diffusible transactivation function establishing the E2 gene product as the diffusible trans-activating factor.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-91
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2990724-Acetyltransferases,
pubmed-meshheading:2990724-Animals,
pubmed-meshheading:2990724-Bovine papillomavirus 1,
pubmed-meshheading:2990724-Cattle,
pubmed-meshheading:2990724-Cell Line,
pubmed-meshheading:2990724-Cell Transformation, Neoplastic,
pubmed-meshheading:2990724-Cell Transformation, Viral,
pubmed-meshheading:2990724-Cercopithecus aethiops,
pubmed-meshheading:2990724-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:2990724-DNA, Recombinant,
pubmed-meshheading:2990724-DNA Replication,
pubmed-meshheading:2990724-Enhancer Elements, Genetic,
pubmed-meshheading:2990724-Genes, Regulator,
pubmed-meshheading:2990724-Genes, Viral,
pubmed-meshheading:2990724-Mice,
pubmed-meshheading:2990724-Papillomaviridae,
pubmed-meshheading:2990724-Plasmids,
pubmed-meshheading:2990724-Promoter Regions, Genetic,
pubmed-meshheading:2990724-Transcription, Genetic,
pubmed-meshheading:2990724-Transfection,
pubmed-meshheading:2990724-Virus Replication
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pubmed:year |
1985
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pubmed:articleTitle |
Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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