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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-8-15
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pubmed:abstractText |
Chemotactic factors stimulate a rapid increase in the cytosolic concentration of intracellular calcium ions ([Ca2+]in) in human polymorphonuclear leukocytes (PMNL), which may be an event that is critical to the expression of chemotaxis and other PMNL functions. Treatment of PMNL with pertussis toxin catalyzes ADP-ribosylation of a protein similar or identical to the inhibiting regulatory protein of adenylate cyclase, Gi, and suppresses the increase in [Ca2+]in elicited by leukotriene B4(LTB4) and formyl-methionyl-leucyl-phenylalanine. Chemotactic migration and lysosomal enzyme release elicited by chemotactic factors were inhibited by pertussis toxin with a concentration-dependence similar to that for inhibition of the increase in [Ca2+]in, without an effect on lysosomal enzyme release induced by the ionophore A23187 and phorbol myristate acetate. Activated pertussis toxin catalyzed the [32P]ADP-ribosylation of a 41 kD protein in homogenates of PMNL. The extent of [32P]ADP-ribosylation of this protein was reduced 59% by pretreatment of intact PMNL with pertussis toxin. Pertussis toxin selectively decreased the number of high-affinity receptors for LTB4 on PMNL by 60% without altering the number or binding properties of the low-affinity subset of receptors. Pertussis toxin modification of a membrane protein of PMNL analogous to Gi thus simultaneously alters chemotactic receptors and attenuates the changes in cytosolic calcium concentration and PMNL function caused by chemotactic factors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-198781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-288052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-293691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-4179066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-4641855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-558197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6091126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6093808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6094010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6100474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6140011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6141069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6142883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6195532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6204890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6209272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6246786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6253447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6254391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6266014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6270168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6293544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6296122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6298231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6300102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6301374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6304074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6308612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6309908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6315733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6323530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6323613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6324930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6330097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-634372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6504157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6770097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6773606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6776111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-6980885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-7015856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-7190221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-7207461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-7342966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-7410854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2989409-901430
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate Ribose,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronidase,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leukotriene B4,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
145-56
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2989409-Humans,
pubmed-meshheading:2989409-Calcium,
pubmed-meshheading:2989409-Glucuronidase,
pubmed-meshheading:2989409-Bacterial Toxins,
pubmed-meshheading:2989409-Neutrophils,
pubmed-meshheading:2989409-Chemotaxis,
pubmed-meshheading:2989409-Cytosol,
pubmed-meshheading:2989409-Receptors, Immunologic,
pubmed-meshheading:2989409-Chemotactic Factors,
pubmed-meshheading:2989409-Adenosine Diphosphate Ribose
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