| pubmed-article:2988256 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C0014432 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:2988256 | lifeskim:mentions | umls-concept:C0205100 | lld:lifeskim |
| pubmed-article:2988256 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2988256 | pubmed:dateCreated | 1985-7-16 | lld:pubmed |
| pubmed-article:2988256 | pubmed:abstractText | A recently described method to investigate growth hormone (GH) receptors on circulating human blood cells has been used to study the effect of a trypsin preparation and antitrypsin moieties on hormone binding to lymphocytes. Trypsinization with one defined dose of trypsin (10 ng/ml) led to a considerable decrease both of specific binding and of binding affinity (affinity constant after 60 min trypsinization 0.5 X 10(9) vs 1.5 X 10(9) M-1 in untreated control cells). Exposure of peripheral blood lymphocytes (PBL) to antitrypsin activities was followed by a steady increase of affinity and specific binding (affinity constant: with 10 KIU anti-trypsin 1.9 X 10(9) M-1, with 100 KIU 2.4 X 10(9) M-1, with 1000 KIU 3.6 X 10(9) M-1). This antitrypsin effect exceeds the binding values expected after blocking trypsin activities in the incubation medium. In a subset of experiments somatomedin-B (SM-B) was used as the antitrypsin moiety and was shown to increase specific GH binding to PBL in a similar manner as did antitrypsin (with 1000 ng SM-B affinity constant (Ka) 12.0 X 10(9) M-1, specific binding 9.7% of total radioactivity). It is concluded that enzymatic factors and their inhibitors including partially GH dependent moieties like SM-B modulate specific GH binding to human peripheral lymphocytes in vitro. | lld:pubmed |
| pubmed-article:2988256 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2988256 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2988256 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2988256 | pubmed:month | May | lld:pubmed |
| pubmed-article:2988256 | pubmed:issn | 0001-5598 | lld:pubmed |
| pubmed-article:2988256 | pubmed:author | pubmed-author:ButenandtOO | lld:pubmed |
| pubmed-article:2988256 | pubmed:author | pubmed-author:KiessWW | lld:pubmed |
| pubmed-article:2988256 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2988256 | pubmed:volume | 109 | lld:pubmed |
| pubmed-article:2988256 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2988256 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2988256 | pubmed:pagination | 139-44 | lld:pubmed |
| pubmed-article:2988256 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:meshHeading | pubmed-meshheading:2988256-... | lld:pubmed |
| pubmed-article:2988256 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2988256 | pubmed:articleTitle | Effect of enzyme and enzyme inhibitors on specific binding of hGH to human peripheral lymphocytes. | lld:pubmed |
| pubmed-article:2988256 | pubmed:publicationType | Journal Article | lld:pubmed |
