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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1985-7-23
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pubmed:abstractText |
We have constructed a restriction map for the genome of bacteriophage MX-8 from Myxococcus xanthus using the enzymes PvuII, MboI, and EcoRI. The phage genome size, as determined by restriction analysis, is 51.7 +/- 0.6 Kb. Double digestions, redigestions of isolated fragments, and crossed-contact hybridization of partial digestion products show that the restriction map is circular. Restriction analysis and Southern hybridization show that the phage DNA molecules are packaged sequentially from a concatemer starting from a specific site which we have mapped. The DNA molecules have an average terminal redundancy of approximately 8% and are circularly permuted over at least 40% of the genome.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0026-8925
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
199
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-32
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2987644-Bacteriophages,
pubmed-meshheading:2987644-Chromosome Mapping,
pubmed-meshheading:2987644-DNA, Circular,
pubmed-meshheading:2987644-DNA, Viral,
pubmed-meshheading:2987644-DNA Restriction Enzymes,
pubmed-meshheading:2987644-Molecular Weight,
pubmed-meshheading:2987644-Myxococcales,
pubmed-meshheading:2987644-Nucleic Acid Conformation
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pubmed:year |
1985
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pubmed:articleTitle |
Physical characterization of the genome of the Myxococcus xanthus bacteriophage MX-8.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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