Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1989-2-7
|
pubmed:abstractText |
A serine protease which catalyses the conversion of the precursor of ANF (atrial natriuretic factor) to the active circulating form, ANF-(99-126)-octacosapeptide, was purified from a particulate fraction of bovine atria. The enzyme was solubilized with a buffer containing 1.6M KCl. The molecular mass of the purified enzyme was 580 kDa on gel filtration, whereas on sodium dodecyl sulfate-polyacrylamide gel electrophoresis a cluster of six bands with molecular masses around 30 kDa was observed. The purified enzyme produced the ANF-(99-126)-octacosapeptide from partially purified bovine pro-ANF by the selective cleavage of the arginyl peptide bond in the -Pro97-Arg98-Ser99-sequence in pro-ANF. It is likely that the enzyme selectively cleaves the Arg98-Ser99 peptide bond in pro-ANF when ANF is secreted into the circulation.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0177-3593
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
369 Suppl
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
113-20
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2974289-Animals,
pubmed-meshheading:2974289-Atrial Natriuretic Factor,
pubmed-meshheading:2974289-Cattle,
pubmed-meshheading:2974289-Chromatography,
pubmed-meshheading:2974289-Chromatography, Gel,
pubmed-meshheading:2974289-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2974289-Heparin,
pubmed-meshheading:2974289-Hydrogen-Ion Concentration,
pubmed-meshheading:2974289-Molecular Weight,
pubmed-meshheading:2974289-Myocardium,
pubmed-meshheading:2974289-Peptide Hydrolases,
pubmed-meshheading:2974289-Protein Precursors,
pubmed-meshheading:2974289-Radioimmunoassay,
pubmed-meshheading:2974289-Spectrometry, Fluorescence
|
pubmed:year |
1988
|
pubmed:articleTitle |
Purification of a specific peptidase in bovine atria for the processing of pro-atrial natriuretic factor.
|
pubmed:affiliation |
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|