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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1988-9-26
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pubmed:abstractText |
Four rat mAb directed against mouse IL-5 have been characterized by their ability to remove and neutralize mouse IL-5 activity in various bioassays. All four mAb absorbed IL-5 activity from solution. Although all were able to neutralize mouse IL-5 bioactivity, two were significantly more effective. These two were also able to neutralize the activity of mouse IL-5 delivered to B cells during a cognate-linked interaction with a Th cell clone. A two-site sandwich ELISA specific for mouse IL-5 was developed by using pairs of mAb. The mouse IL-5 ELISA is capable of detecting natural or mouse rIL-5 in supernatants, crude bacterial lysates, and high concentrations of mouse serum, and has a detection limit of less than 20 pg. Two of these antibodies cross-reacted with and neutralized human rIL-5, and one of these was used for development of an ELISA for human IL-5.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1576-81
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2970507-Animals,
pubmed-meshheading:2970507-Antibodies, Monoclonal,
pubmed-meshheading:2970507-Antibody Specificity,
pubmed-meshheading:2970507-Antigen-Antibody Complex,
pubmed-meshheading:2970507-Blood Chemical Analysis,
pubmed-meshheading:2970507-Clone Cells,
pubmed-meshheading:2970507-Cross Reactions,
pubmed-meshheading:2970507-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2970507-Female,
pubmed-meshheading:2970507-Humans,
pubmed-meshheading:2970507-Hybridomas,
pubmed-meshheading:2970507-Immunosorbents,
pubmed-meshheading:2970507-Interleukin-5,
pubmed-meshheading:2970507-Interleukins,
pubmed-meshheading:2970507-Mice,
pubmed-meshheading:2970507-Mice, Inbred BALB C,
pubmed-meshheading:2970507-Neutralization Tests,
pubmed-meshheading:2970507-Rats,
pubmed-meshheading:2970507-Rats, Inbred Lew,
pubmed-meshheading:2970507-T-Lymphocytes, Helper-Inducer
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pubmed:year |
1988
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pubmed:articleTitle |
The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent.
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pubmed:affiliation |
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304-1140.
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pubmed:publicationType |
Journal Article
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