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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-7-1
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pubmed:abstractText |
The 25 kDa mRNA cap binding protein can be purified in a partially phosphorylated state and the extent of its phosphorylation appears to be regulated during heat shock and mitosis in mammalian cells. We demonstrated that a nonabundant serine protein kinase activity exists in rabbit reticulocytes that phosphorylates the 25 kDa cap binding protein in both the free (eIF-4E) and complexed (eIF-4F) state. This kinase was not inhibited by the cAMP-dependent protein kinase inhibitory peptide IAAGRTGRRNAIHDILVAA, did not phosphorylate S6 ribosomal protein, did not phosphorylate p220 of eIF-4F as protein kinase C does and no other substrates for this kinase were apparent in reticulocyte ribosomal salt wash. The molecular identity of this kinase, the specific site(s) of eIF-4E that it phosphorylates and its in vivo regulatory role remain to be studied.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
153
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
340-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2967701-Animals,
pubmed-meshheading:2967701-Carrier Proteins,
pubmed-meshheading:2967701-Eukaryotic Initiation Factor-4E,
pubmed-meshheading:2967701-Eukaryotic Initiation Factor-4F,
pubmed-meshheading:2967701-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:2967701-Peptide Initiation Factors,
pubmed-meshheading:2967701-Phosphorylation,
pubmed-meshheading:2967701-Protein Kinases,
pubmed-meshheading:2967701-Protein-Serine-Threonine Kinases,
pubmed-meshheading:2967701-RNA Cap-Binding Proteins,
pubmed-meshheading:2967701-RNA Caps,
pubmed-meshheading:2967701-Rabbits,
pubmed-meshheading:2967701-Reticulocytes
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pubmed:year |
1988
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pubmed:articleTitle |
Identification of a protein kinase activity in rabbit reticulocytes that phosphorylates the mRNA cap binding protein.
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pubmed:affiliation |
Department of Medicine (Gastroenterology) and Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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