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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1987-4-29
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pubmed:abstractText |
Insulin treatment (Kact, 5 X 10(-9) M) of serum-starved 3T3-L1 adipocytes stimulates a soluble serine/threonine kinase that catalyzes phosphorylation of microtubule-associated protein 2 (MAP-2) in vitro. Maximal activation of MAP-2 kinase activity by 80 nM insulin was observed after 10 min of hormonal stimulation, prior to maximal stimulation of S6 kinase activity (20 min). The insulin-stimulatable MAP-2 kinase activity is not adsorbed to phosphocellulose, whereas the principal S6 kinase activity is retained and elutes at approximately 0.5 M NaCl. The insulin-stimulatable MAP-2 kinase is less stable during incubation at 30 degrees C than S6 kinase activity. Inclusion of phosphatase inhibitors decreases the rate at which the stimulated MAP-2 kinase activity is lost from extract supernatants incubated at 30 degrees C. p-Nitrophenyl phosphate is more effective than DL-phosphotyrosine, whereas DL-phosphoserine is without effect at the concentration used (40 mM). The difference in MAP-2 kinase activity in extract supernatants from control and insulin-treated cells is also preserved after rapid chromatography on Sephadex G-25. These results show that a soluble serine/threonine kinase is rapidly activated by insulin, possibly by phosphorylation of either the kinase itself or an interacting modulator.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-1259145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-202261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-214578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-2991283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-2992967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-3011539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-3511906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-3514207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-36738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-3892533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-3920212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-457745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-4853976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-543535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-6149966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-6167574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-6196602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-6196603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-6294652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-6313661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2951732-81205
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1502-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2951732-Adipose Tissue,
pubmed-meshheading:2951732-Amino Acids,
pubmed-meshheading:2951732-Cells, Cultured,
pubmed-meshheading:2951732-Dose-Response Relationship, Drug,
pubmed-meshheading:2951732-Enzyme Activation,
pubmed-meshheading:2951732-Insulin,
pubmed-meshheading:2951732-Microtubule-Associated Proteins,
pubmed-meshheading:2951732-Nitrophenols,
pubmed-meshheading:2951732-Organophosphorus Compounds,
pubmed-meshheading:2951732-Phosphorylation,
pubmed-meshheading:2951732-Protein Kinases,
pubmed-meshheading:2951732-Protein-Serine-Threonine Kinases
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pubmed:year |
1987
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pubmed:articleTitle |
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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