Linked Life Data

2943626

Source:http://linkedlifedata.com/resource/pubmed/id/2943626

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-6
pubmed:dateCreated
1986-10-20
pubmed:abstractText
Natural murine interferon-gamma (IFN-gamma) produced by the T lymphoma, L12R4, stimulated with phorbol myristic acetate was purified by anti-murine IFN-gamma immunoadsorbent and labeled with 125I to study its binding to receptors of mouse cells. By adding increasing concentrations of unlabeled natural murine IFN-gamma in competition binding assays we determined a KD of 8.2 X 10(-10) M for L1210 cells and a number of receptors of about 3000. Moreover protease, but not endoglycosidase treatment of target cells prevented the subsequent binding of the ligand. These studies demonstrate that natural murine IFN-gamma binds in a specific manner and with high affinity to receptors on murine cell lines.
pubmed:language
ita
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0390-5462
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
70-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
[Analysis of the gamma interferon receptor in mice].
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't