| pubmed-article:2934386 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C0025248 | lld:lifeskim |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C0031621 | lld:lifeskim |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C0041217 | lld:lifeskim |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:2934386 | lifeskim:mentions | umls-concept:C1883712 | lld:lifeskim |
| pubmed-article:2934386 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2934386 | pubmed:dateCreated | 1986-2-12 | lld:pubmed |
| pubmed-article:2934386 | pubmed:abstractText | The variant surface glycoproteins (VSGs) of Trypanosoma brucei are synthesized with a hydrophobic COOH-terminal peptide that is cleaved and replaced by a glycophospholipid, which anchors VSG to the surface membrane. The kinetics of VSG processing were studied by metabolic labeling with [35S]methionine and [3H]myristic acid. The COOH-terminal oligosaccharide-containing structure remaining after phospholipase removal of dimyristyl glycerol from membrane-form VSG could be detected serologically within 1 min of polypeptide synthesis in two T. brucei variants studied. Addition of the oligosaccharide-containing structure was resistant to tunicamycin. VSGs synthesized in the presence of tunicamycin displayed lower apparent molecular weights, consistent with the complete inhibition of N-glycosylation at one (variant 117), two (variant 221), or at least three (variant 118) internal asparagine sites. In most experiments, N-glycosylation appeared to occur during or immediately after polypeptide synthesis but in a few cases N-glycosylation was delayed or incomplete. In all cases, addition of the COOH-terminal oligosaccharide-containing structure occurred normally. In dual-labeling studies, cycloheximide caused rapid inhibition of both [35S]methionine and [3H]myristic acid incorporation, suggesting that myristic acid addition also occurs immediately after polypeptide synthesis. Our data suggest that the complex ethanolamine-glycosyl-dimyristylphosphatidylinositol structure of membrane-form VSG is added en bloc within 1 min of completion of the polypeptide. | lld:pubmed |
| pubmed-article:2934386 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2934386 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2934386 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2934386 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2934386 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2934386 | pubmed:author | pubmed-author:CrossG AGA | lld:pubmed |
| pubmed-article:2934386 | pubmed:author | pubmed-author:OverathPP | lld:pubmed |
| pubmed-article:2934386 | pubmed:author | pubmed-author:FergusonM AMA | lld:pubmed |
| pubmed-article:2934386 | pubmed:author | pubmed-author:DuszenkoMM | lld:pubmed |
| pubmed-article:2934386 | pubmed:author | pubmed-author:LamontG SGS | lld:pubmed |
| pubmed-article:2934386 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2934386 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:2934386 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:2934386 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2934386 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2934386 | pubmed:pagination | 356-62 | lld:pubmed |
| pubmed-article:2934386 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:meshHeading | pubmed-meshheading:2934386-... | lld:pubmed |
| pubmed-article:2934386 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:2934386 | pubmed:articleTitle | Biosynthesis of Trypanosoma brucei variant surface glycoproteins. N-glycosylation and addition of a phosphatidylinositol membrane anchor. | lld:pubmed |
| pubmed-article:2934386 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2934386 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2934386 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2934386 | lld:pubmed |
