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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1985-12-4
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pubmed:abstractText |
Cells of a human monocyte-like cell line (U937) were analysed for IgE Fc receptors (Fc epsilon R) before and after glucocorticoid treatment. Specific binding of human myeloma IgE (Sha) was measured by 125I-labelled IgE, and by fluorescein-labelled IgE monitored by flow cytometry. Treatment of cells with dexamethasone or other steroids with glucocorticoid activity caused a significant decrease in Fc epsilon R expression. The inhibition was dose dependent, with a half-maximal effect at 20 nM dexamethasone, a concentration which is near to the dissociation constant of glucocorticoid receptors for dexamethasone. Inhibition of Fc epsilon R was significant beginning 8 h following glucocorticoid treatment and reached a plateau at 24 hr. The Ka for IgE binding was similar for control and dexamethasone-treated cells, while the number of IgE binding sites was decreased by 50-60%. Culture supernatants from dexamethasone-treated U937 cells which were concentrated 10-fold and depleted of free steroid did not affect Fc epsilon R expression. These results demonstrate that glucocorticoids can directly decrease the number of Fc epsilon R. This effect could participate in the glucocorticoid-induced suppression of IgE-mediated allergic reactions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-109532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-112109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-1184745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-321474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-41058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-450087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6088628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6166866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6192145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6211489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6214406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6220071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6223089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6254391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6759607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6838573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-6972964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-7014719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-7061861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-7190991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-7197722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-7285390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932386-872868
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
359-66
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2932386-Antibodies, Monoclonal,
pubmed-meshheading:2932386-Antigens, Surface,
pubmed-meshheading:2932386-Binding Sites, Antibody,
pubmed-meshheading:2932386-Cell Line,
pubmed-meshheading:2932386-Dexamethasone,
pubmed-meshheading:2932386-Dose-Response Relationship, Drug,
pubmed-meshheading:2932386-Humans,
pubmed-meshheading:2932386-Immunoglobulin E,
pubmed-meshheading:2932386-Monocytes,
pubmed-meshheading:2932386-Receptors, Fc,
pubmed-meshheading:2932386-Receptors, IgE,
pubmed-meshheading:2932386-Receptors, Immunologic,
pubmed-meshheading:2932386-Time Factors
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pubmed:year |
1985
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pubmed:articleTitle |
Glucocorticoids inhibit IgE receptor expression on the human monocyte cell line U937.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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