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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3 Pt 1
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pubmed:dateCreated |
1989-4-14
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pubmed:abstractText |
The previously developed chelator O-aminophenol-N,N,O-triacetic acid (APTRA) (L. A. Levy, E. Murphy, B. Raju, and R. E. London. Biochemistry 27: 4041-4048, 1988) has been modified to yield a fluorescent analogue which can be utilized as an intracellular probe for ionized Mg2+. The fluorescent analogue, FURAPTRA, with a magnesium dissociation constant of 1.5 mM, is structurally analogous to the calcium chelator fura-2 and exhibits a similar excitation shift on magnesium complexation. Hence, data on the intracellular Mg2+ concentration can be obtained using an analogous ratio method. The acetoxymethyl form of the chelator is readily loaded into cells and has been used to determine a cytosolic free Mg2+ concentration of 0.59 mM for isolated rat hepatocytes. As a consequence of the relatively high levels of cytosolic Mg2+, the problem of ion buffering is much less severe than for the analogous calcium indicators.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Oxazoles
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C540-8
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:2923192-Animals,
pubmed-meshheading:2923192-Benzofurans,
pubmed-meshheading:2923192-Cells, Cultured,
pubmed-meshheading:2923192-Cytosol,
pubmed-meshheading:2923192-Fluorescent Dyes,
pubmed-meshheading:2923192-Fura-2,
pubmed-meshheading:2923192-Indicators and Reagents,
pubmed-meshheading:2923192-Liver,
pubmed-meshheading:2923192-Magnesium,
pubmed-meshheading:2923192-Male,
pubmed-meshheading:2923192-Oxazoles,
pubmed-meshheading:2923192-Rats,
pubmed-meshheading:2923192-Rats, Inbred Strains,
pubmed-meshheading:2923192-Spectrometry, Fluorescence
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pubmed:year |
1989
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pubmed:articleTitle |
A fluorescent indicator for measuring cytosolic free magnesium.
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pubmed:affiliation |
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
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pubmed:publicationType |
Journal Article
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