rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
1989-4-7
|
pubmed:abstractText |
The NH2-terminal blocking group of the 43-kDa peripheral membrane protein (43-kDa protein) of Torpedo post-synaptic membranes has been identified as myristic acid. To identify that blocking group pure 43-kDa protein was digested with trypsin and the blocked tryptic peptide was isolated by reverse phase HPLC. That peptide coeluted with and had the same amino acid composition as a synthetic peptide, myristoyl-Gly-Gln-Asp-Gln-Thr-Lys, the structure of the amino terminus predicted from the protein sequence deduced from a cDNA clone. The presence of myristate was confirmed by the precise molecular mass of the peptide, 886.5266, determined by fast atom bombardment mass spectroscopy.
|
pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0014-5793
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
16
|
pubmed:volume |
243
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
65-9
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2920827-Amino Acids,
pubmed-meshheading:2920827-Animals,
pubmed-meshheading:2920827-Glycoproteins,
pubmed-meshheading:2920827-Mass Spectrometry,
pubmed-meshheading:2920827-Molecular Weight,
pubmed-meshheading:2920827-Myristic Acid,
pubmed-meshheading:2920827-Myristic Acids,
pubmed-meshheading:2920827-Oligopeptides,
pubmed-meshheading:2920827-Peptide Fragments,
pubmed-meshheading:2920827-Receptors, Nicotinic,
pubmed-meshheading:2920827-Synaptic Membranes,
pubmed-meshheading:2920827-Torpedo,
pubmed-meshheading:2920827-Trypsin
|
pubmed:year |
1989
|
pubmed:articleTitle |
Myristic acid is the NH2-terminal blocking group of the 43-kDa protein of Torpedo nicotinic post-synaptic membranes.
|
pubmed:affiliation |
Department of Anatomy, Washington University School of Medicine, St. Louis, MO 63110.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|