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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-2-14
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pubmed:abstractText |
The human alpha 1-globin gene was fused downstream of two erythroid-specific DNase I super-hypersensitive sites that are normally located upstream of the human beta-globin locus. This construct was injected into fertilized mouse eggs, and expression was analyzed in 16-day fetal livers and brains. All 11 fetuses that contained intact copies of the transgene expressed correctly initiated human alpha-globin mRNA in the erythroid fetal liver but not in brain. Levels of expression ranged from 4% to 337% of endogenous mouse beta-globin mRNA. A human alpha-globin construct that did not contain super-hypersensitive sites was not expressed. These results demonstrate that human beta-globin locus activation sequences can stimulate high levels of human alpha-globin gene expression in erythroid tissue of transgenic mice. The results also provide a foundation for experiments designed to coexpress human alpha- and beta-globin genes in transgenic mice and suggest a feasible approach for production of a mouse model for human sickle cell disease.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-2992937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3018541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3337825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3456593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3480506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3545063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3690667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3719696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3731277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3858676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3868484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3879975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3892534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-3982505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-4066658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6200874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6205764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6277502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6313205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6313220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6325149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6446404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6572107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6578416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911581-6946454
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2911581-Animals,
pubmed-meshheading:2911581-Blotting, Southern,
pubmed-meshheading:2911581-Brain,
pubmed-meshheading:2911581-Fetus,
pubmed-meshheading:2911581-Gene Expression Regulation,
pubmed-meshheading:2911581-Genes,
pubmed-meshheading:2911581-Globins,
pubmed-meshheading:2911581-Humans,
pubmed-meshheading:2911581-Liver,
pubmed-meshheading:2911581-Mice,
pubmed-meshheading:2911581-Mice, Inbred C57BL,
pubmed-meshheading:2911581-Mice, Transgenic,
pubmed-meshheading:2911581-Organ Specificity,
pubmed-meshheading:2911581-RNA, Messenger,
pubmed-meshheading:2911581-Transcription, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
High-level erythroid expression of human alpha-globin genes in transgenic mice.
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pubmed:affiliation |
Department of Biochemistry, Schools of Medicine and Dentistry, University of Alabama, Birmingham 35294.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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