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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1987-12-9
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pubmed:abstractText |
As part of an analysis of the conjugative transfer genes associated with the expression of F pili by plasmid F, we have investigated the physical location of the traC and traW genes. We found that plasmid clones carrying a 2.95-kilobase EcoRI-EcoRV F transfer operon fragment were able to complement transfer of F lac traC mutants and expressed an approximately 92,000-dalton product that comigrates with TraC. We also found that traW-complementing activity was expressed from plasmids carrying a 900-base-pair SmaI-HincII fragment. The traW product was identified as an approximately 23,000-dalton protein. The two different F DNA fragments that expressed traC and traW activities do not overlap. Our data indicate that the traC gene is located in a more-tra operon promoter-proximal position than suggested on earlier maps and that traW is distal to traC. These results resolve a long-standing question concerning the relationship of traW to traC. The clones we have constructed are expected to be useful in elucidating the role of proteins TraC and TraW in F-pilus assembly.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-1108020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-2827204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-2861807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-2880557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-2885308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-2940611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-3040671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-346570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-372967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-383387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-4555412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-4929865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-6111288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-6120442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-6145171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-6219273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-6279869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-6300946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2889720-745238
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5119-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2889720-Bacterial Proteins,
pubmed-meshheading:2889720-Cloning, Molecular,
pubmed-meshheading:2889720-Escherichia coli,
pubmed-meshheading:2889720-F Factor,
pubmed-meshheading:2889720-Fimbriae, Bacterial,
pubmed-meshheading:2889720-Genes,
pubmed-meshheading:2889720-Genes, Bacterial,
pubmed-meshheading:2889720-Genetic Vectors,
pubmed-meshheading:2889720-Mutation,
pubmed-meshheading:2889720-Operon,
pubmed-meshheading:2889720-Plasmids
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pubmed:year |
1987
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pubmed:articleTitle |
Location of F plasmid transfer operon genes traC and traW and identification of the traW product.
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pubmed:affiliation |
Department of Medical Microbiology and Immunology, Texas A & M University, College Station 77843.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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