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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1987-8-17
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pubmed:abstractText |
Midguts of mosquitoes, Aedes aegypti and Anopheles stephensi, and of the tsetse fly, Glossina morsitans morsitans, as well as guinea pig pancreas, were prepared for electron microscopy by using low-temperature embedding in Lowicryl K4M. Rabbit antiserum to bovine pancreatic polypeptide (PP) crossreacted with secretory granules of pancreatic PP-producing cells and of the clear cells in mosquito gut. Rabbit antiserum to human somatostatin crossreacted with the control tissue, guinea pig pancreas D-cells, but not with the mosquito clear cells. None of the antisera used showed a distinct reaction with the endocrine-like cells of tsetse fly midgut. Positive reactions were revealed by gold as electron-dense marker. The gold particles were coated with protein A-gold or goat antibodies to rabbit immunoglobulin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1554
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
891-6
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:2885369-Aedes,
pubmed-meshheading:2885369-Animals,
pubmed-meshheading:2885369-Anopheles,
pubmed-meshheading:2885369-Endocrine Glands,
pubmed-meshheading:2885369-Epithelium,
pubmed-meshheading:2885369-Gold,
pubmed-meshheading:2885369-Immunologic Techniques,
pubmed-meshheading:2885369-Intestinal Mucosa,
pubmed-meshheading:2885369-Microscopy, Electron,
pubmed-meshheading:2885369-Pancreatic Polypeptide,
pubmed-meshheading:2885369-Somatostatin,
pubmed-meshheading:2885369-Tsetse Flies
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pubmed:year |
1987
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pubmed:articleTitle |
Immunoelectron microscopic demonstration of pancreatic polypeptide in midgut epithelium of hematophagous dipterans.
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pubmed:publicationType |
Journal Article
|