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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1987-2-6
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pubmed:abstractText |
Interrelation between the effects of C-protein and LC2-light chains on actin-activated ATPase activity of skeletal muscle myosin has been investigated at various ionic strength (0.06-0.14) and free calcium levels (10(-4) M, 10(-8) M). The ATPase activity of AM reconstituted with column-purified myosin or partly-purified myosin and non-regulated actin exhibits a pronounced dependence on ionic strength with maximum at I = 0.1. C-protein impurities (5 per cent) usually present in Minit can inhibit AM ATPase at every ionic strength assayed, without changing the character of this dependence. Actin-activated ATPase of the above myosins shows calcium sensitivity at every ionic strength studied. The partial removal of LC2 from Mcol results in a decrease of AM ATPase and in a disappearance of its calcium sensitivity. C-protein added to Mcol in a molar ratio of 1:1 inhibits considerably AM ATPase, reduces its sensitivity to ionic strength and abolishes its calcium sensitivity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myosins,
http://linkedlifedata.com/resource/pubmed/chemical/citrate-binding transport protein
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pubmed:status |
MEDLINE
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pubmed:issn |
0237-6261
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
21
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
247-56
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2879405-Adenosine Triphosphatases,
pubmed-meshheading:2879405-Animals,
pubmed-meshheading:2879405-Calcium,
pubmed-meshheading:2879405-Carrier Proteins,
pubmed-meshheading:2879405-Muscles,
pubmed-meshheading:2879405-Myosins,
pubmed-meshheading:2879405-Osmolar Concentration,
pubmed-meshheading:2879405-Rabbits
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pubmed:year |
1986
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pubmed:articleTitle |
Effect of C-protein and LC-light chains on actomyosin ATPase at various ionic strength and calcium levels.
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pubmed:publicationType |
Journal Article,
In Vitro
|