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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1986-12-10
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pubmed:abstractText |
The isolation of dengue viruses from clinical specimens has always posed a particularly difficult problem. The use of invertebrate cell cultures such as AP-61 and C6/36 has reduced the time required for definitive diagnosis to within a week. More recently, inoculation of adult mosquitoes has been used but it requires more than a week to reach a confirmed laboratory diagnosis. We describe a method using intracerebral inoculation of immobilized fourth instar of Toxorhynchites splendens larvae for the isolation of dengue viruses from clinical specimens which yields results within a few days following incubation at 32 degrees C.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
|
pubmed:issn |
0166-0934
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
133-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2877004-Animals,
pubmed-meshheading:2877004-Antigens, Viral,
pubmed-meshheading:2877004-Culicidae,
pubmed-meshheading:2877004-Dengue Virus,
pubmed-meshheading:2877004-Fluorescent Antibody Technique,
pubmed-meshheading:2877004-Humans,
pubmed-meshheading:2877004-Larva,
pubmed-meshheading:2877004-Temperature
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pubmed:year |
1986
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pubmed:articleTitle |
Isolation of dengue viruses by intracerebral inoculation of mosquito larvae.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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