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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1979-9-1
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pubmed:abstractText |
Male CD-1 mice were orally treated with 0.01, 0.1, 1 and 10 micrograms TCDD/kg body wt./week for up to 8 weeks. Randomly selected animals were sacrificed at 2, 4, and 8 weeks of exposure. An additional group was given orally 10 micrograms TCDD/kg and the animals similarly sacrificed. Splenic lymphocytes from these animals were cultured in vitro with or without the presence of phytomitogens, phytohemagglutinin, and pokeweed mitogen. The incorporation of 3H-thymidine was measured as an indication of relative blast formation. Exposure of animals to TCDD, even at the lowest level (0.01 microgram/kg/wk for 2 weeks) caused a marked increase in the thymidine uptake by cultured lymphocytes. The blastogenic response of mitogens was reduced at high levels of TCDD exposure, indicating an immunosuppressive effect. Following a single treatment with 10 micrograms TCDD/kg, the increase in the blast formation was noted at 2 weeks, the effect reduced at 4 weeks, and no difference noticed in treated vs. control cultures 8 weeks after the treatment. Small doses of TCDD stimulate the splenic lymphocyte transformation and this effect, although somewhat dose-related, can be reversed in a relatively short period of time.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0077-8923
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
320
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
487-97
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:287401-Animals,
pubmed-meshheading:287401-Body Weight,
pubmed-meshheading:287401-Cells, Cultured,
pubmed-meshheading:287401-DNA,
pubmed-meshheading:287401-Dioxins,
pubmed-meshheading:287401-Immune Tolerance,
pubmed-meshheading:287401-Liver,
pubmed-meshheading:287401-Lymphocyte Activation,
pubmed-meshheading:287401-Lymphocytes,
pubmed-meshheading:287401-Male,
pubmed-meshheading:287401-Mice,
pubmed-meshheading:287401-Mitogens,
pubmed-meshheading:287401-Organ Size,
pubmed-meshheading:287401-Spleen,
pubmed-meshheading:287401-Tetrachlorodibenzodioxin,
pubmed-meshheading:287401-Thymus Gland
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pubmed:year |
1979
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pubmed:articleTitle |
Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on splenic lymphocyte transformation in mice after single and repeated exposures.
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pubmed:publicationType |
Journal Article
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