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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1989-6-5
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pubmed:databankReference | |
pubmed:abstractText |
Two overlapping clones, covering the entire coding sequence of human M2-type pyruvate kinase (PK) cDNA, were isolated and sequenced. Nucleotide sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
509-16
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2854097-Amino Acid Sequence,
pubmed-meshheading:2854097-Base Sequence,
pubmed-meshheading:2854097-Blotting, Southern,
pubmed-meshheading:2854097-Carcinoma, Hepatocellular,
pubmed-meshheading:2854097-Chromosome Mapping,
pubmed-meshheading:2854097-Chromosomes, Human, Pair 15,
pubmed-meshheading:2854097-Cloning, Molecular,
pubmed-meshheading:2854097-DNA, Recombinant,
pubmed-meshheading:2854097-Genes,
pubmed-meshheading:2854097-Humans,
pubmed-meshheading:2854097-Isoenzymes,
pubmed-meshheading:2854097-Liver Neoplasms,
pubmed-meshheading:2854097-Molecular Sequence Data,
pubmed-meshheading:2854097-Nucleic Acid Hybridization,
pubmed-meshheading:2854097-Pyruvate Kinase,
pubmed-meshheading:2854097-Restriction Mapping
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pubmed:year |
1988
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pubmed:articleTitle |
Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma.
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pubmed:affiliation |
Department of Internal Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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