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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-3-21
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pubmed:abstractText |
Several putative plasma-membrane-associated components of the T-lymphocyte signal-transduction pathway are phosphorylated during the initial events of cellular activation. Little is known about the control of dephosphorylation of these components. We have shown by immunoblotting that the type 1 phosphatase, the type 2A phosphatase and type 2B phosphatase (calcineurin) are associated with the plasma membrane of normal human T lymphoblasts and the human T leukaemic cell line Jurkat 6. The type 1 phosphorylase phosphatase activity is present in a latent form which can be stimulated synergistically by deinhibitor and p-nitrophenyl phosphate. The PCSH form of the type 2A phosphatase appears to be the predominant oligomer in the plasma-membrane fraction. All three phosphatases can be extracted from membranes with Nonidet P40, but whereas the type 1c and type 2Ac phosphatases separate into the detergent-poor phase of Triton X-114, calcineurin separates into both detergent-rich and -poor phases. It is probable that one or more of these three plasma-membrane-associated phosphatases play regulatory roles in determining the phosphorylation state of membrane-bound proteins involved in human T-cell activation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-13538949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-211869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2416335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2437014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2444644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2822491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2826150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2827745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2830909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2841890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2845955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2880725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2946683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2983609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2984199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2985385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2986124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-2989299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3007140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3013843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3015619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3021755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3027074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3027075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3030282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3037526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3040407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3040474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3100962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3112151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3114259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3116077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3257504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3426556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3494729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3611055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3871355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-3934668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-4321928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-4444531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-4611687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6089515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6254981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6257680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6280056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6306765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6319140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6323426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6426470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6576338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-6721859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-7144578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-7160476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2852007-962102
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
885-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
The association of type 1, type 2A and type 2B phosphatases with the human T lymphocyte plasma membrane.
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pubmed:affiliation |
Imperial Cancer Research Fund, London, U.K.
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