Linked Life Data

2848220

Source:http://linkedlifedata.com/resource/pubmed/id/2848220

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1989-1-5
pubmed:abstractText
The expression of Herpes Simplex Virus 1 (HSV-1) glycoprotein C (gC), a well defined herpesvirus late gene, was studied by linking the promoter-regulatory region of this gene to the coding sequences for the bacterial enzyme, beta-galactosidase (beta-gal). A chimeric gene, containing the beta-gal gene under the control of gC sequences from -1350 to +30 relative to the mRNA start site, was inserted by homologous recombination into the thymidine kinase (TK) locus of the HSV-1 genome. Selection of the TK- recombinant virus by plaque assay was facilitated by addition of a beta-gal indicator to the agarose overlay. Recombinant virus containing the gC promoter-beta-gal chimeric gene faithfully expressed beta-gal as a viral late gene, as shown by the absence of beta-gal expression when viral DNA replication was inhibited with phosphonoacetic acid. In contrast, the inhibition of viral DNA replication had no effect on the expression of beta-gal when the beta-gal gene was under the control of the early HSV-1 TK promoter in a separate recombinant virus. Analysis of recombinant viruses containing 5' to 3' deletions in the gC regulatory region revealed no apparent difference in beta-gal expression as deletions extended from -1350 to -109 base-pairs (bp) before the RNA start site, demonstrating that sequences between -109 and +30 are sufficient for regulated gC expression in the viral genome. Analysis of the mRNA made by these recombinant viruses confirmed the results of the beta-gal assays, and demonstrated that the transcriptional start sites of the gC promoter-beta-gal chimeric genes were the same as the start site of the gC gene.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-225559, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2824847, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2833425, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2983322, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2985955, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2991561, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2991596, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2993644, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-2993649, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-3004024, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-3018926, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-3021980, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-3025606, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-3031620, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-3939316, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-4365321, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-4373710, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-4833542, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6088791, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6091116, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6096556, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6235151, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6246451, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6254015, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6258155, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6262799, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6277739, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6283634, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6294341, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6300426, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6302308, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6314251, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6317894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6325170, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6325883, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6328000, http://linkedlifedata.com/resource/pubmed/commentcorrection/2848220-6330737
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10267-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The use of beta-galactosidase as a marker gene to define the regulatory sequences of the herpes simplex virus type 1 glycoprotein C gene in recombinant herpesviruses.
pubmed:affiliation
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't