Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1988-11-21
pubmed:abstractText
We have constructed simple test-plasmids to study transcriptional enhancers in yeast. In this paper the reporter-gene is a plasmid borne deletion-substitution derivative (his-del4) of the Saccharomyces cerevisiae HIS3 gene in which the native promoter has been replaced by a dormant, susceptible pseudopromoter. We investigate the function in yeast of foreign control elements, the polyomavirus enhancer and some of its derivatives, inserted in either orientation at the 3' or 5' ends of the reporter gene. The polyoma enhancer (and, although less thoroughly studied, the SV40 enhancer) will strongly activate transcription from latent start sites within the pseudo-promoter sequence. The rules we draw for the polyoma enhancer effect in yeast are, with a few interesting exceptions, remarkably similar to those discovered by experimentation in mammalian cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2419129, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2834068, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2836064, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2838739, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2882858, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2982105, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2982495, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2985964, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2989541, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-2991922, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3001645, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3011279, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3018552, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3018583, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3037380, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3459589, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3909105, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3909145, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3909147, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-3912167, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6095089, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6096017, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6096863, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6096864, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6173489, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6173490, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6259625, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6275354, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6287274, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6305503, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6305591, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6305592, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6323032, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-6354471, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845360-7142162
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8847-68
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Foreign transcriptional enhancers in yeast. I. Interactions of papovavirus transcriptional enhancers and a quiescent pseudopromoter on supercoiled plasmids.
pubmed:affiliation
International Institute of Genetics and Biophysics (CNR), Naples, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't