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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1988-11-23
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pubmed:abstractText |
A single vaccination of mice with a complex of porin and smooth lipopolysaccharide (porin-S-LPS) extracted from virulent Brucella abortus 2308 provided significant protection (P less than 0.01 to P less than 0.001) against challenge with the same strain, equivalent to that achieved by vaccination with living attenuated B. abortus 19. The porin-S-LPS vaccine given without adjuvant or in several adjuvants (trehalose dimycolate and muramyl dipeptide; the pluronic polymer L-121 and muramyl dipeptide; or complexed with Quil A in immunostimulating complexes) provided equivalent protection. In contrast, one vaccination with porin complexed with rough LPS (porin-R-LPS) from a rough mutant of strain 2308 provided no protection with any adjuvant tested. In one experiment, two inoculations with the porin-R-LPS resulted in a low level of protection, probably owing to priming of the animals for production of O-polysaccharide-specific antibodies. However, one vaccination with rough-strain porin covalently bound to purified O polysaccharide conferred protection equal to that obtained with natural complexes of porin-S-LPS or with living strain 19. A synthetic vaccine containing long chains of O polysaccharide was more effective than one prepared with short chains. Protective vaccines caused the formation of increased concentrations of circulating O-polysaccharide-specific antibodies, although there were individual exceptions to the quantitative association between O-polysaccharide-specific antibodies and protection. Antibodies specific for porin or R-LPS were found in negligible quantities in vaccinated mice. These results provide additional evidence that the O polysaccharide will constitute an essential component of an effective subcellular vaccine against B. abortus and that O-polysaccharide-specific antibodies play an important role in protective immunity in brucellosis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-101226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-108257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-121112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-14194388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-14392312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-2469929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-2469935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3005173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3033095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3089933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3115279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3125668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3133872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3354257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3499713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3546142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-387596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-387597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3932211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3937324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-3937731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-413471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6203984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6207248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6315592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6323316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6333362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6356268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6408007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6436094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6437981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6437982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6489617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6491284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6709052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6757218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6758425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6802061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6802764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6805054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-680804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6814766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6980838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6982229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-6995612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-7035868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-7264301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2844673-750371
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Porins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2808-17
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2844673-Animals,
pubmed-meshheading:2844673-Mice,
pubmed-meshheading:2844673-Brucellosis,
pubmed-meshheading:2844673-Vaccines,
pubmed-meshheading:2844673-Vaccination,
pubmed-meshheading:2844673-Brucella abortus,
pubmed-meshheading:2844673-Polysaccharides, Bacterial,
pubmed-meshheading:2844673-Bacterial Vaccines,
pubmed-meshheading:2844673-Glycolipids,
pubmed-meshheading:2844673-Adjuvants, Immunologic
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