Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1988-10-6
pubmed:abstractText
We used a Southern hybridization assay to locate precisely the sites at which DNA replication is arrested in the terminus region of the Escherichia coli chromosome. The assay was based on the properties of restriction fragments that contain stalled replication forks. Replication forks that entered the terminus from the clockwise direction with respect to the genetic map were inhibited near manA at a site called T2, which we located at kilobase 442 on the physical map of Bouché (J. P. Bouché, J. Mol. Biol. 154:1-20, 1982). Those that entered the terminus region traveling in the counterclockwise direction were inhibited near pyrF at a site called T1, which we located at kilobase 90. In each case we found only a single, precise site of arrest. Inhibition at T1 was not detectable in our assay in strains lacking the trans-acting locus tus, which is located near T2 and is required for T1 to function. We demonstrated that the sites of inhibition are also used during termination of replication in exponentially growing, wild-type cells. In all previous studies on the terminus of E. coli, inhibition has only been detected in strains that were modified so that the origin used was placed near the terminus to force the use of the sites of inhibition.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-16453826, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3038334, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3039353, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3119858, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3156375, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3276664, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-333449, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-338909, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3550796, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-3550797, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-6094834, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-6096020, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-6273909, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-6281436, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-6348505, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842310-7262563
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4293-8
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Location of sites that inhibit progression of replication forks in the terminus region of Escherichia coli.
pubmed:affiliation
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.